Coding

Part:BBa_K4932003:Design

Designed by: Zofia Ziemkiewicz, Edward Harris   Group: iGEM23_Oxford   (2023-10-03)


LucKey


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 283
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 774
    Illegal PstI site found at 283
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 283
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 283
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

If you are designing your own protein, currently (2023) it only really works well with small, alpha helical targets.


Source

https://www.ipd.uw.edu/wp-content/uploads/2021/01/Rubio_et_al_Nature_COVID_LOCKR_sensors.pdf

For the whole labelled plasmid, please see

The sequence provided is just the coding sequence for the LucCage protein but in our guides we will be using our whole plasmid. We could not get the igem system to accept the labels for this.

References

Alfredo Quiiano-Rubio et al 2021 https://www.ipd.uw.edu/wp-content/uploads/2021/01/Rubio_et_al_Nature_COVID_LOCKR_sensors.pdf